Browsing Molecular & Structural Biology by Title

Now showing items 1-20 of 83

Abnormal erythrocyte membrane phospholipid organisation in chronic myeloid leukaemia

Kumar, Ajay; Daniel, S; Agarwal, S S; Gupta, C M (1987)

The membrane phospholipid organisation in the red cells of humans suffering from chronic myeloid leukaemia has been analysed using the amino-group labelling reagent trinitrobenzenesulphonic acid and the nuid-sensing ...
An actin-like protein is involved in regulation of mitochondrial and flagellar functions as well as in intramacrophage survival of Leishmania donovani

Singh, Kuldeep; Veluru, N K; Trivedi, Vishal; Gupta, C M; Sahasrabuddhe, A A (2014)

Actin related proteins are ubiquitous actin-like proteins that show high similarity with actin in terms of their amino acid sequence and three-dimensional structure. However, in lower eukaryotes, such as trypanosomatids, ...
Antibody-mediated targeting of Liposomes to erythocytes in whole blood

Singhal, Arun; Bali, Anu; Gupta, C M (1986)

F(ab)2 fragments derived from anti-rat erythocyte antibody or normal rabbit serum lgG were covalently attached to the surface of liposomes of equimolar amounts egg phoshatidylcholine and cholesterol. These liposomes were ...
Antibody-mediated targeting of liposomes to red cells in vivo

Singhal, Arun; Gupta, C M (1986)

Covalent attachment of anti-rat erythrocyte F(ab')2 to liposomes specifically enhanced their binding to rat erythrocytes in vivo and reduced their uptake by the liver. Furthermore, at least 20-30% of the cell-bound liposomes ...
Asymmetric Distribution of Phosphatidylethanolamine in C. albicans: Possible Mediation by CDRI, a Multidrug Transporter Belonging to ATP Binding Cassette (ABC) Superfamily

Dogra, S; Krishnamurthy, S; Gupta, V; Dixit, B L; Gupta, C M; Sanglard, D; Prasad, R (1999)

By using two molecular probes, we demonstrate that only 4% of total phosphatidylethanolamine (PtdEtn) in the plasma membrane (PM) of a human pathogenic yeast, Candida albicans, is present in its external half. Evidence is ...
Biochemical and Biophysical Characterization of Leishmania donovani Gamma-glutamylcysteine Synthetase

Agnihotri, Pragati; Singh, S P; Shakya, A K; Pratap, J V (2016)

γ-glutamylcysteine synthetase (Gcs) is a vital enzyme catalyzing the first and rate limiting step in the trypanothione biosynthesis pathway, the ATP-dependent ligation of L-Glutamate and L-Cysteine to form gamma-glutamylcysteine. ...
Biophysical Characterization of Entamoeba histolytica Phosphoserine aminotransferase (EhPSAT): Role of Cofactor and Domains in Stability and Subunit Assembly

Mishra, Vibhor; Ali, Vahab; Nozaki, Tomoyoshi; Bhakuni, Vinod (2011)

We have investigated the role of cofactor PLP, and its binding domain in stability and subunit assembly of Phosphoserine aminotransferase (EhPSAT) from an enteric human parasite Entamoeba histolytica. The presence of ...
Carbamyl Analogs of Phosphatidylcholines Synthesis, Interaction With Phospholipases and Permeability Behavior of their Liposomes

Gupta, C M; Bali, Anu (1981)

A novel class of phospholipase-resisting phosphatidylcholine analogs, in which the C-2 ester group or both C-1 and C-2 ester groups have been replaced by carbamyloxy functions (-NH-C-O-), have been synthesized. These lipids ...
Characterization of antimicrobial, cytotoxic and anti-endotoxin properties of short peptides with different hydrophobic amino acids at ‘a’ and ‘d’ positions of a heptad repeat sequence

Azmi, Sarfuddin; Srivastava, Saurabh; Mishra, N N; Tripathi, J K; Shukla, P K; Ghosh, J K (2013)

To understand the influence of different hydrophobic amino acids at ‘a’ and ‘d’ positions of a heptad repeat sequence on antimicrobial, cytotoxic and anti-endotoxin properties, four fifteen-residues peptides with leucine ...
Characterization of hyaluronic acid specific hyaluronate lyase (HylP) from Streptococcus pyogenes

Singh, S K; Malhotra, Soniya; Akhtar, M S (2014)

Streptococcus pyogenes is associated with a wide variety of mucosal and invasive infections that claim human life. The conversion from non pathogenic to toxigenic strain of S. pyogenes are thought to be mediated by ...
Cloning, overexpression, purification and preliminary X-ray analysis of a Feast/famine regulatory protein (Rv2779c) from Mycobacterium tuberculosis H37Rv

Dey, Abhishek; Ramachandran, Ravishankar (2014)

Rv2779c from M. tuberculosis is a Feast/Famine regulatory protein also known as Leucine responsive regulatory protein/Asparagine synthase C- family (Lrp/AsnC) of transcriptional regulators. This class of proteins is known ...
Crystal structure of the Hexachlorocyclohexane dehydrochlorinase (LinA-type2): mutational analysis, thermostability & enantioselectivity

Macwan, A S; Kukshal, Vandna; Srivastava, Nidhi; Javed, Saleem; Kumar, Ashwani; Ramachandran, Ravishankar (2012)

Hexachlorocyclohexane dehydrochlorinase (LinA) mediates dehydrochlorination of -HCH to 1, 3, 4, 6-tetrachloro-1,4-cyclohexadiene that constitutes first step of the aerobic degradation pathway. We report the 3.5 Å crystal ...
Design and characterization of short antimicrobial peptides using leucine zipper templates with selectivity towards microorganisms

Ahmad, Aqeel; Azmi, Sarfuddin; Srivastava, Saurabh; Kumar, Amit; Tripathi, J K; Mishra, N N; Shukla, P K; Ghosh, J K (2014)

Design of antimicrobial peptides with selective activity towards microorganisms is an important step towards the development of new antimicrobial agents. Leucine zipper sequence has been implicated in cytotoxic activity ...
Design of non-toxic analogs of cathelicidin-derived bovine anti-microbial peptide BMAP–27: The role of leucine as well as phenylalanine zipper sequences in determining its toxicity

Ahmad, Aqeel; Azmi, Sarfuddin; Srivastava, R M; Srivastava, Saurabh; Pandey, B K; Saxena, Rubha; Bajpai, V K; Ghosh, J K (2009)

BMAP-27 is a cathelicidin-derived bovine anti-microbial peptide, which shows moderate cytotoxicity and potent antibacterial activity against a wide variety of microorganisms. Despite a number of studies, very little is ...
DNA topoisomerase 2 mutant allele mildly delays the mitotic progression and activates the checkpoint protein kinase Chk1 in fission yeast

Yadav, Sudhanshu; Verma, S K; Ahmed, Shakil (2011)

DNA topoisomerases are specialized nuclear enzymes that perform topological modifications on double stranded DNA and hence are essential for DNA metabolism such as replication, transcription, recombination, condensation ...
Effect of Altered Polar Headgroup of Phosphatidylethanolamines on Transbilayer Aminophospholipid Distribution in Sonicated Vesicles

Kumar, Ajay; Gupta, C M (1983)

The transbilayer aminophospholipid distributions in small unilamellar vesicles comprising of phosphatidylethanolamine or its analogs (bearing modifications in the polar headgroup) and egg hosphatidylcholine were ascertained ...
The effect of fusidic acid on Plasmodium falciparum elongation factor G (EF-G)

Gupta, Ankit; Mir, S S; Saqib, Uzma; Biswas, Subir; Vaishya, Suniti; Srivastava, Kumkum; Siddiqi, M I; Habib, Saman (2013)

Inhibition of growth of the malaria parasite Plasmodium falciparum by known translation-inhibitory antibiotics has generated interest in understanding their action on the translation apparatus of the two genome containing ...
Enhanced Immunostimulant Activity and Protective Effect of a Synthetic Lipopeptide after Liposomization Against Plasmodium berghei Infection in Mice.

Agrawal, Ajay K; Puri, A; Haq, W; Gupta, C M (2001)

The immunostimulant activity of non-pyrogenic, sugar-free immunomodulator lipopeptide, Ala-D-Glu(Gly-Lys-Co.C11H23)-NH2 (comp. no.84/201), and its liposomized formulation has been studied. Liposomization of this lipopeptide ...
Enhancement in anti-Semliki Forest virus activity of ds RNA by a muramyl dipeptide

George, C X; Jain, Rakesh K; Gupta, C M; Anand, Nitya (1986)

Antiviral activity of an interferon-inducing mycoviral ds RNA against Semliki Forest virus infection was considerably enhanced by N-palmitoylmuramyl-L-alanyl-D-isoglutamine (PMDP), a new muramyl dipeptide. This enhancement ...
FLAG tag module for PCR based gene targeting

Ranjan, Rajeev; Verma, S K; Ahmed, Shakil (2010)

Epitope tagging of yeast proteins has become an efficient tool for biochemical analysis of protein of interest. The epitope-tagged proteins can be used for western blotting, immunoprecipitation and immunofluorescence ...

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