Abstract:
Conditions have been standardized for the estimation of 4 : 4' -diaminodiphenyl sulfide in the presence of biological materials and its metabolism has been studied. The drug is absorbed gradually over a long period and is distributed fairly uniformly in all the tissues, except the brain, and it does not seem to be able to cross the blood-brain barrier. The main route of excretion is the kidneys. The drug circulates in the blood mainly in the form of a polar conjugate of 4 : 4'diaminodiphenyl
sulfide. The major metabolites in the rabbit and human urine were 4: 4' -diaminodiphenyl sulfide, the corresponding sulfoxide, sulfone and a polar unidentified compound.
A method has been standardized for the salting out chromatography of 4: 4'diaminodiphenyl sulfide, sulfoxide and sulfone; and the method is particularly
suitable for sulfides, since these give rather high and unpredictable Rf values by
conventional paper partition chromatography.·
