• Th1 stimulatory proteins of Leishmania donovani: Comparative cellular and protective responses of recombinant-triose phosphate isomerase, protein disulfide isomerase, and elongation factor-2, in combination with recombinant HSP70 against visceral leishmaniasis 

  Jaiswal, A K; Khare, Prashant; Joshi, Sumit; Kushawaha, P K; Sundar, Shyam; Dube, Anuradha (2014)

  In visceral leishmaniasis, the recovery from the disease is always associated with the generation of Th1-type of cellular responses. Based on this, we have previously identified several Th1-stimulatory proteins of Leishmania ...
• Molecular cloning and biochemical characterization of iron superoxide dismutase from the rodent malaria parasite Plasmodium vinckei 

  Prakash, Kirtika; Goyal, Manish; Soni, Awakash; Siddiqui, A J; Bhardwaj, Jyoti; Puri, S K (2014)

  Plasmodium parasite utilizes superoxide dismutase family proteins to limit the toxicity of reactive oxygen species, such as produced through hemoglobin degradation. These proteins play an important role in parasite survival ...
• Potential Cerebral malaria therapy: Intramuscular arteether and vitamin D co-administration 

  DWIVEDI, HEMLATA; SINGH, SK; CHAUHAN, BS; GUNJAN, S; TRIPATHI, R (2016)

  Cerebral malaria (CM) shows lethality rate of 15-25% despite effective antimalarial chemotherapy. The effective adjunct treatment to counteract the CM pathogenesis is urgently required. In murine cerebral malaria model, ...
• Wolbachia endosymbiont of Brugia malayi elicits Th-17 mediated proinflammatory immune response through surface protein (WSP) 

  Pathak, Manisha; Verma, Meenakshi; Srivastava, Mrigank; Bhattacharya-Misra, Shailja (2014)

  Wolbachia is an endosymbiotic bacterium of filarial nematode Brugia malayi. The symbiotic relationship between Wolbachia and its filarial host is dependent on some interactions between the proteins of both organisms. ...
• Cloning, overexpressionand characterization of soluble 42 kDa fragment of merozoite surface protein-1 of Plasmodium vivax 

  Sheikh, I H; Kaushal, D C; Singh, Vandana; Kumar, Niraj; Chandra, Deepak; Kaushal, N A (2014)

  Plasmodium vivax represents the second most prevalent malaria species of major public health importance and the global eradication of malaria requires the development of vaccines to prevent infection. The lack of in vitro ...
• Immunological evaluation of an rsmD-like rRNA methyltransferase from Wolbachia endosymbiont of Brugia malayi 

  Rana, A K; Kushwaha, Susheela; Singh, P K; Bhattacharya-Misra, Shailja (2016)

  Wolbachia is a wonderful anti-filarial target with many of its enzymes and surface proteins (WSPs) representing potential drug targets and vaccine candidates. Here we report on the immunologic response of a drug target, ...
• Moxidectin causes adult worm mortality of human lymphatic filarial parasite Brugia malayi in rodent models 

  Verma, Meenakshi; Pathak, Manisha; Shahab, Mohd; Singh, Kavita; Mitra, Kalyan; Bhattacharya-Misra, Shailja (2014)

  Moxidectin is a macrocyclic lactone belonging to milbemycin family closely related to ivermectin and is currently progressing towards Phase III clinical trial against human infection with the filaria Onchocerca volvulus ...
• Nitroimidazo-oxazole Compound (DNDI-VL-2098) - An Orally Effective Preclinical Drug Candidate for the Treatment of Visceral Leishmaniasis 

  Gupta, Suman; Yardley, Vanessa; Vishwakarma, Preeti; Shivahare, Rahul; Sharma, Bhawna; Launay, Delphine; Martin, Denis; Puri, S K (2015)

  Objective: Identification of a nitroimidazo-oxazole lead molecule for the treatment of visceral leishmaniasis (VL). Methods: A library of 72 nitroimidazo-oxazoles was evaluated in vitro for their antileishmanial activity ...
• Structural modelling studies and immunoprophylactic potential of Brugia malayi DEAD Box RNA Helicase 

  Singh, Meghna; Shrivastava, Nidhi; Saqib, Uzma; Siddiqi, M I; Bhattacharya-Misra, Shailja (2013)

  DEAD Box RNA helicases are essential enzymes that are involved in RNA metabolic processes such as, transcription, pre-mRNA splicing, translation initiation, and RNA decay. We had earlier overexpressed and biochemically ...
• Cloning, Expression and Characterization of UDP-N-acetylglucosamine enolpyruvyl transferase (MurA) from Wolbachia Endosymbiont of Human Lymphatic Filarial Parasite Brugia malayi 

  Shahab, Mohd; Verma, Meenakshi; Pathak, Manisha; Mitra, Kalyan; Bhattacharya-Misra, Shailja (2014)

  Wolbachia, an endosymbiont of filarial nematode, is considered a promising target for treatment of lymphatic filariasis. Although functional characterization of the Wolbachia peptidoglycan assembly has not been fully ...
• Wolbachia translation initiation factor-1 is copiously expressed by the adult, microfilariae and infective larvae of Brugia malayi and competitively inhibited by tetracycline 

  Nag, J K; Shrivastava, Nidhi; Tiwari, Manish; Gupta, C L; Bajpai, Preeti; Chahar, Dhanvantri; Bhattacharya-Misra, Shailja (2014)

  The intracellular alphaproteobacteria, Wolbachia, is considered to be a future antimacrofilarial drug target as it is obligatory for filarial endurance. Characterizing wolbachial proteins is necessary to understand wolbachial ...
• Molecular characterization of an rsmD-like rRNA methyltransferase from Wolbachia endosymbiont of Brugia malayi and antifilarial activity of specific inhibitors of the enzyme 

  Rana, A K; Chandra, Sharat; Siddiqi, M I; Bhattacharya-Misra, Shailja (2013)

  The endosymbiotic Wolbachia of Brugia malayi are an attractive antifilarial drug target. These bacteria possess an mutualistic relationship with the filarial parasite and impair filarial growth and survival if treated with ...
• Nucleosomal Histone Proteins of L. donovani: A combination of recombinant H2A, H2B, H3 and H4 proteins were highly immunogenic and offered optimum prophylactic efficacy against Leishmania challenge in hamsters 

  Baharia, R K; Tandon, Rati; Sahasrabuddhe, A A; Sundar, Shyam; Dube, Anuradha (2014)

  The present study includes cloning and expression of recombinant L.donovani histone proteins (rLdH2B, rLdH3, rLdH2A and rLdH4), assessment of their immunogenicity in Leishmania infected cured patients/endemic contacts as ...
• Design, Synthesis, ADME Characterization and Antileishmanial Evaluation of Novel Substituted Quinoline Analogs 

  Gopinath, V S; Mukkavilli, Rao; Shivahare, Rahul; Vishwakarma, Preeti; Ghose, Sweta; Pradhan, Ashok; Hindupur, Ramamohan; Sarma, K D; Gupta, Suman; Puri, S K; Launay, Delphine; Martin, Denis (2014)

  In vitro ADME characterization of the lead compound 1 identified for visceral leishmaniasis was undertaken and further structural analogs were synthesized for antileishmanial screening. Compound 1 was highly permeable in ...
• Wolbachia Transcription Elongation Factor “Wol GreA” Interacts With α2ββ’σ Subunits of RNA Polymerase Through Its Dimeric C-terminal Domain 

  Nag, J K; Shrivastava, Nidhi; Chahar, Dhanvantri; Gupta, C L; Bajpai, Preeti; Misra-Bhattacharya, Shailja (2014)

  Objectives: Wolbachia, an endosymbiont of filarial nematode, is considered a promising target for therapy against lymphatic filariasis. Transcription elongation factor GreA is an essential factor that mediates transcriptional ...
• Immunization of Mastomys coucha with Brugia malayi recombinant trehalose-6-phosphate phosphatase results in significant protection against homologous challenge infection 

  Kushwaha, Susheela; Singh, P K; Rana, A K; Misra-Bhattacharya, Shailja (2013)

  Development of a vaccine to prevent or reduce parasite development in lymphatic filariasis would be a complementary approach to existing chemotherapeutic tools. Trehalose-6-phosphate phosphatase of Brugia malayi (Bm-TPP) ...
• Disorganized muscle protein-1 (DIM-1) of filarial parasite Brugia malayi: cDNA cloning, expression, purification, structural modeling and its potential as vaccine candidate for human filarial infection 

  Kushwaha, Vikas; Kumar, Vikash; Verma, S K; Sharma, Rolee; Siddiqi, M I; Murthy, P K (2014)

  We have recently identified disorganized muscle protein-1 (DIM-1) in one of the proinflammatory fractions of the human filaria Brugia malayi adult worm. The present study was undertaken to characterize B. malayi DIM-1 ...
• Setaria Cervi Antigen Fraction For Serodiagnosis Of Human Lymphatic Filariasis By Dot-Elisa 

  Priyadarshi, Priyanka; Kaushal, D C; Tandon, Ashish; Ali, Shakir; Kaushal, N A (2014)

  A partially purified antigen fraction of Setaria cervi was used in Dot-enzyme linked immunosorbent assay (Dot-ELISA) for detection for filaria–specific antibodies in W. bancrofti infected patient sera. The S. cervi adult ...
• Combination of Liposomal CpG Oligodeoxynucleotide 2006 and Miltefosine Induces Strong Cell-Mediated Immunity during Experimental Visceral Leishmaniasis 

  Shivahare, Rahul; Vishwakarma, Preeti; Parmar, Naveen; Yadav, P K; Haq, Wahajul; Srivastava, Mrigank; Gupta, Suman; Kar, Susanta (2014)

  Immuno-modulators in combination with antileishmanial drug miltefosine is a better therapeutic approach for treatment of Visceral Leishmaniasis (VL) as it not only reduces the dose and toxicity of miltefosine but also ...
• Modulation of host’s immune responses and parasite load in Mastomys coucha immunized with recombinant mitochondrial HSP60 of human lymphatic filarial parasite Brugia malayi 

  Verma, A K; Verma, S K; Kushwaha, Vikas; Verma, Richa; Siddiqui, W A; Murthy, P K (2013)

  Recently we have shown that mitochondrial HSP60 of Brugia malayi (mtHSP60bm) shows high degree of homology with Escherichia coli GroEL/ES and that the ATP binding pockets of HSP60 in humans, E. coli and B. malayi were ...

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