Abstract:
A partially purified antigen fraction of Setaria cervi was used in Dot-enzyme linked immunosorbent assay (Dot-ELISA) for detection for filaria–specific antibodies in W. bancrofti infected patient sera. The S. cervi adult antigen extract was fractionated by gel filtration on Sephacryl S-500 column and antigen fractions (obtained just after the void volume) showed high reactivity with polyclonal antibodies against S. cervi excretory-secretory fraction-I (having potential for immunodiagnosis of human filariasis) as well as with filarial patient serum pools.. The partially purified S. cervi antigen fraction (ScAS-1) was used in Dot ELISA for detection of antibodies in filarial patient sera. The Dot-ELISA was optimised in terms of optimum antigen concentration, antibody dilutions and secondary antibody conjugate and 50 ng of antigen per dot, serum dilution of 1:250 and 1:1000 dilution of secondary antibody conjugate were found optimal for Dot ELISA. The optimised Dot-ELISA was evaluated using 30 filarial patients sera, 20 sera with other parasitic diseases and 20 normal healthy individuals. The partially purified S. cervi antigen fraction could detect anti-filarial antibodies in filarial patients sera by Dot-ELISA.
