Antidiabetic and Hypolipidemic Activity in Stem of Jatropha Gossypifolia L.

Abstract

Objectives- The present study carried out to investigate the antidiabetic and hypolipidemic efficacy of active fractions (chloroform fraction of 95% ethanolic extract and butanol fraction of aqueous extract) of J.gossypifolia L.(stem) by measuring the status of blood glucose, serum insulin, carbohydrate metabolizing enzymes, lipid levels, renal and hepatic parameters on diabetic rats. Methods- Chloroform fraction of 95% ethanolic extract and butanol fraction of aqueous extract of J.gossypifolia L. (stem) were administered to diabetic groups daily up to 15 days. Blood glucose levels of the diabetic rats were monitored at intervals of 3 hours for acute study and 15 days for prolonged study. Biochemical parameters notably fasting blood sugar, glucose tolerance, lipid profile, renal and hepatic functions markers and enzymatic activity were assessed. Results- In single dose study, chloroform fraction of 95% ethanolic extract and butanol fraction of aqueous extract of J.gossypifolia L.(stem), significantly reduced blood glucose levels of diabetic rats while in multiple dose studies, chloroform fraction of 95% ethanolic extract restored the elevated biochemical parameters significantly to the near normal level in type 2 diabetic rats when compared with standard drug Metformin (100 mg/kg) but butanol fraction of aqueous extract did not show any marked effect. Conclusion- The present study thus concludes that the antidiabetic efficacy of chloroform fractions of the 95% ethanolic extract of J.gossypifolia L.(stem) have favourable effect in bringing down the severity of hyperglycemia, enhancing hypolipidemic activity, modulating effect on carbohydrate metabolizing enzymes, decline the increased level of renal and hepatic function markers and also improving glucose tolerance activity.