Rv3080c regulates the rate of inhibition of mycobacteria by Isoniazid through FabD

Kumari, Ruma; Saxena, Richa; Tiwari, Sameer; Tripathi, D K; Srivastava, K K

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dc.contributor.author	Kumari, Ruma
dc.contributor.author	Saxena, Richa
dc.contributor.author	Tiwari, Sameer
dc.contributor.author	Tripathi, D K
dc.contributor.author	Srivastava, K K
dc.date.accessioned	2013-03-08T09:50:57Z
dc.date.available	2013-03-08T09:50:57Z
dc.date.issued	2013
dc.identifier.citation	Molecular and Cellular Biochemistry 2013, 374, (1-2) 149-155	en
dc.identifier.uri	http://hdl.handle.net/123456789/1029
dc.description.abstract	The mycobacterial FAS-II multienzyme complex has been identified to be a target of Ser/Thr protein kinases (STPKs) of Mycobacterium tuberculosis (MTB), with substrates, including the malonyl-CoA:ACP transacylase (FabD) and the β-ketoacyl-ACP synthases KasA and KasB. These proteins are phosphorylated by various kinases in vitro. The present study links the correlation of FASII pathway with serine threonine protein kinase of MTB. In the preliminary finding we have shown that mycobacterial protein Rv3080c (PknK) phosphorylates FabD and the knockdown of PknK protein in mycobacteria down regulates FabD expression. This event leads to the differential inhibition of mycobacteria in presence of Isoniazid (INH), as the inhibition of growth of mycobacteria in presence of INH is enhanced in PknK deficient mycobacteria.	en
dc.format.extent	693573 bytes
dc.format.mimetype	application/pdf
dc.language.iso	en	en
dc.relation.ispartofseries	CDRI Communication No. 8347	en
dc.subject	Mycobacteria	en
dc.subject	PknK	en
dc.subject	FabD	en
dc.subject	FasII	en
dc.title	Rv3080c regulates the rate of inhibition of mycobacteria by Isoniazid through FabD	en
dc.type	Article	en