Abstract:
Fifty-eight basic dyes have been examined for their suitability as reagents for paper chromatographic detection of condensed phosphates. Of these dyes, toluidine blue has been found to be the reagent of choice because of its sensitivity and relative specificity. Another advantage of the use of toluidine blue is that it permits the recovery of the detected compound (free from the reagent) by a simple and mild procedure. The reaction between this dye and the polyanions on filter paper is adversely influenced by a large number of organic and inorganic cations.
