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| Title: | Determination of 3-Hydroxy pterocarpan, a novel osteogenic compound in rat plasma by liquid chromatography-tandem mass spectrometry: application to pharmacokinetics study |
| Authors: | Manickavasagama, Lakshmi
Gupta, Sonal
Mishra, Smriti
Kumar, A
Raghuvanshi, Ashutosh
Goel, Atul
Singh, Divya
Jain, G K |
| Keywords: | Pterocarpans
Isoflavones
Pharmacokinetics
Rat plasma |
| Issue Date: | 2011 |
| Citation: | Biomedical chromatography (2011), 25(7), 843-50 |
| Series/Report no.: | CDRI COMMUNICATION NO.7957 |
| Abstract: | A rapid, sensitive and selective LC-MS/MS method for the quantitative analysis of 3-hydroxy pterocarpan (S006-1709) in female rat plasma has been developed and validated. Discovery RP18 column was used for the chromatographic elution using acetonitrile and 0.1% acetic acid in water as mobile phase (80: 20 v/v) at the flow rate of 0.5ml/min. MS/MS analysis was performed in triple quadrupole mass spectrometer with electrospray ionization (ESI) in negative ion mode using biochanin as an internal standard (IS). Extraction of S006-1709 and IS from rat plasma was done by liquid-liquid extraction method using diethyl ether. The LC-MS/MS method was sensitive with 1.95ng/ml as the limit of detection (LOD) and 3.9ng/ml as lower limit of quantification (LLOQ). The method was linear in the concentration range of 3.9ng/ml to 1000ng/ml. The % bias for intraday and interday accuracy was not greater than 4.2 and the % R.S.D for intraday and interday precision was not greater than 13.2. The recovery of S006-1709 and IS were 73.9 to 79.3 % and 85.7% respectively. S006-1709 was found to be stable in various stability studies. The validated LC-MS/MS method was successfully applied for the oral pharmacokinetics study of S006-1709 at 10mg/kg in female S.D. rats. |
| URI: | http://hdl.handle.net/123456789/689 |
| Appears in Collections: | Pharmacokinetics & Metabolism
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| gkjain-7957-biomedical chromatography(2011), 25(7), 843-50.pdf | | 327Kb | Adobe PDF | View/Open |
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