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http://hdl.handle.net/123456789/206
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| Title: | Targeted cytosolic delivery of hydrogel nanoparticles into HepG2 cells through engineered Sendai viral envelopes |
| Authors: | Jana, Siddhartha S
Bharali, Dhruba J
Mani, Prashant
Maitra, Amarnath
Gupta, C M
Sarkar, Debi P |
| Keywords: | Hydrogel nanoparticle
Sendai virus
Virosome
Drug delivery
Targeted cytosolic delivery
Controlled release |
| Issue Date: | 2002 |
| Citation: | FEBS Letters 515, 184- 189 |
| Abstract: | Hydrogel nanoparticles of cross-linked polyvinylpyrrolidone (PVP-NP) (35-50 nm in diameter) containing fluoresceinated dextran (FITC-Dx) were encapsulated in reconstituted Sendai viral envelopes containing only the fusion (F) protien (F- virosomes). Incubtion of these loaded F- virosmes with human hepatoblastoma cells (HepG2) in culture cytoplasm, as inferred froom the ability of cells tointernalize FITC-Dx loaded PVP-NP(PVP-NP) in the presence of azide (an inhibitor of the endocytotic process). Introduction of PVP-NP into the HelpG2 cells wasw assured by selctive accumulation of FITC fluorescence in the cytosolic compartment. The structural integrity of the internalzed PVP-NP was also confirmed by fluorescence microscopy and ultracentrifugation analysis. The potential usefulness of PVP-NP -mediated cytosolic release of water soluble drugs boyh in vitro and in vivo has been established for the first time. |
| URI: | http://hdl.handle.net/123456789/206 |
| Appears in Collections: | Molecular & Structural Biology
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| cmg-2002-febslett-515-184-189pdf.pdf | | 2987Kb | Adobe PDF | View/Open |
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