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| Title: | Effect of Altered Polar Headgroup of Phosphatidylethanolamines on Transbilayer Aminophospholipid Distribution in Sonicated Vesicles |
| Authors: | Kumar, Ajay
Gupta, C M |
| Keywords: | Phospholipid distribution
Phosphatidylethanolamine analog
Aminophospholipid
Headgroup
Membrane vesicle |
| Issue Date: | 1983 |
| Citation: | Biochimica el Biophysica Acta (1983), 730, 1-9 |
| Series/Report no.: | CDRI Communication No. 2718 |
| Abstract: | The transbilayer aminophospholipid distributions in small unilamellar vesicles comprising of phosphatidylethanolamine
or its analogs (bearing modifications in the polar headgroup) and egg hosphatidylcholine were ascertained using trinitrobenzenesulfonic acid as external membrane probe. These vesicles, containing
10-30 mol% phosphatidylethanolamine or its analogs, were formed by sonication and fractionated by centrifugation. Phosphatidylethanolamine at low concentrations (10 mol%) preferentially localized in the outer monolayer. This preference appeared to be reversed at higher phosphatidylethanolamine concentrations (30 mol%). Unlike this finding, phosphatidylethanolamine bearing ethyl, phenyl and benzyl substituents at the
carbon atom adjacent to the amino group distributed mainly in the outer surface irrespective of their concentrations. Similar results were obtained when the phosphate and amino groups were separated by three methylene residues. These observations suggest that the effective polar headgroup volume and/or
hydrogen-bonding capacity of phospholipids are the important factors that determine their distribution in small unilamellar vesicles. |
| URI: | http://hdl.handle.net/123456789/114 |
| Appears in Collections: | Molecular & Structural Biology
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| cmg-1983-BBA-730-1.pdf | | 3436Kb | Adobe PDF | View/Open |
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