http://dkr.cdri.res.in:8080/xmlui/handle/123456789/698 Sun, 19 Apr 2026 13:38:13 GMT 2026-04-19T13:38:13Z http://dkr.cdri.res.in:8080/xmlui/handle/123456789/1652 Callus culture and in vitro biosynthesis of echitamine from Alstonia scholaris (L.) R.Br. Singh, S K; Joshi, Trapti; Kanojiya, Sanjeev; Tripathi, Vineeta; Mishra, D K Alstonia scholaris (L.) R. Br., belonging to the family Apocynaceae, is a rich source of indole alkaloids among which echitamine is the most important. It is reported as an antiproliferative agent and targeted for cancer chemotherapy. However, echitamine and its derivatives are mainly concentrated in stem bark and root of the plant and bulk collection of these parts from nature is not recommended. The present study is the first attempt to standardize the induction and proliferation of callus from leaf explants of A. scholaris along with in vitro biosynthesis of echitamine. Quantitative estimation of echitamine was performed by using ultra performance liquid chromatography mass spectrometry. The medium used for callus induction and proliferation was Murashige and Skoog which was optimized with various combinations and concentrations of different auxins and cytokinins. Best induction and proliferation of callus was noted in 2,4-dichlorophenoxyacetic acid (2,4-D) and 6-furfurylamino purine (FAP) combination with their specific concentration at 0.5:0.5 mg L-1. Furthermore, the data indicated that both auxin/cytokinin ratio as well as their independent concentration was important for the same. Echitamine biosynthesis was observed in 0.5:0.5 and 0.5:0.3 mg L-1 of 2,4-D and FAP under 16:8 h light-dark cycle. However, production of echitamine was increased more than two folds in 0.5:0.3 mg L-1 of 2,4-D and FAP containing medium upon application of yeast extract at 150 mg L-1 with five days incubation period. Thus, in vitro biosynthesis may offer an alternative source of echitamine without harming natural plant population. Thu, 01 Jan 2015 00:00:00 GMT http://dkr.cdri.res.in:8080/xmlui/handle/123456789/1652 2015-01-01T00:00:00Z http://dkr.cdri.res.in:8080/xmlui/handle/123456789/1633 Ethanolic extract of Coelogyne cristata Lindley (Orchidaceae) and its compound coelogin promote osteoprotective activity in ovariectomized estrogen deficient mice Sharma, Chetan; Mansoori, M N; Dixit, Manisha; Shukla, Priyanka; Kumari, Tejaswita; Bhandari, S P S; Narender, T; Singh, Divya; Aryaa, K R Coelogyne cristata Lindley (CC) family Orchidaceae is an Indian medicinal plant used for the treatment of fractured bones in folk- tradition of Kumaon region, Uttarakhand, India. In continuation of our drug discovery program, feeding of ethanolic extract to ovariectomized estrogen deficient mice led to significant restoration of trabecular micro architecture in both femoral and tibial bones, better bone quality and also devoid of any uterine estrogenicity. Subsequently, coelogin, a pure compound was isolated from ethyl acetate fraction of C. cristata and evaluated in in-vitro osteoblast cell cultures. Treatment of coelogin to osteoblasts led to enhanced ALP activity (a marker of osteoblast differentiation), mineral nodule formation and mRNA levels of osteogenic markers like BMP-2, Type 1 Collagen and RUNX-2. Based on these results, we propose that ethanolic extract of Coelogyne cristata and its pure compound coelogin have potential in the management of post menopausal osteoporosis. Wed, 01 Jan 2014 00:00:00 GMT http://dkr.cdri.res.in:8080/xmlui/handle/123456789/1633 2014-01-01T00:00:00Z http://dkr.cdri.res.in:8080/xmlui/handle/123456789/1445 Qualitative determination of bioactive metabolites through Q-TOF LC/MS in different parts and undifferentiated cultures of Ulmus wallichiana Planchon Kumari, Tejaswita; Sharma, Chetan; Bajpai, Vikas; Kumar, Brijesh; Srivastava, Mukesh; Arya, K R Ulmus wallichiana (UW) is an endangered and folk medicinal plant used for healing fractures in India. Recently, the secondary metabolites isolated and identified from its bark revealed osteogenic activity. Considering the medicinal properties of its bark and the source for its identified and isolated metabolites, the requirement for production of alternative pathways was crucial. This study aimed to determine its bioactive metabolite using Q-TOF LC/MS (Quadrupole time-of-flight liquid chromatography- mass spectroscopy) in different parts of this plant with seasonal and altitudinal variability pertaining with the development of a protocol for induction of callus cultures and biomass production. Plant samples were collected during December - March, April - July and August - November in the year 2010-2012 from three different altitudinal locations (Nainital, Almora and Bageshwar districts) of Kumaon region, Uttarakhand, India. The results obtained from Q-TOF LC/MS analysis demonstrated the presence of four bioactive metabolites known for osteogenic activity in bark and stem samples round the year. Syntheses of bioactive metabolites in leaf samples were significantly affected by sampling season as well as altitudinal locations respectively. Callus derived from leaf explants was maintained in Murashige & Skoog (MS) medium containing (0.25 mgl-1) 2,4-Dichlorophenoxy-acetic acid (2,4-D) + (0.05 mgl-1 ) Kinetin (Kn) and (0.25 mgl-1) α-Naphthaleneacetic acid (NAA) + (0.5 mgl-1) 6- Benzylaminopurine (BAP) in liquid phase showed optimal conditions for accumulating biomass and synthesis of identified bioactive metabolites. Thu, 01 Jan 2015 00:00:00 GMT http://dkr.cdri.res.in:8080/xmlui/handle/123456789/1445 2015-01-01T00:00:00Z http://dkr.cdri.res.in:8080/xmlui/handle/123456789/1377 Development of quality control markers for Ulmus wallichiana Planchon: A Indian traditional plant for osteogenic activity Arya, K R; Khatoon, Sayyada; Kumar, Brijesh Ulmus wallichiana Planchon, is an Indian folk traditional plant used for the treatment of fractured bones in folklore tradition of Uttarakhand Himalaya, India. During chemical investigations, three major compounds, Ulmoside A & B, and Naringenin-6-C-β-D glucopyranosideis for osteogenic activity have been isolated from bark of this plant species. This paper presents macro and microscopic study, physicochemical parameters and chemo-profiling of stem bark of U. wallichiana through Q TOF HRMS for development of quality control markers and identification of crude samples. Transverse section (TS) stem bark and powder microscopy shows crushed rhytidoma consisting plenty of mucilaginous canals followed by crushed cork, cells of the cork filled with dark brown content. Whereas, authenticity and quality of raw materials can be determinate with exact calculated mass value of marker compounds for osteogenic activity at 467, 451 and 435 using Q TOF HRMS technique. Ursolic acid, β-sitosterol and lupeol at Rf value 0.25, 0.36 and 0.44 has also identified as HPTLC marker for identification and authentication of crude drug samples of stem bark of U. wallichiana. Tue, 01 Jan 2013 00:00:00 GMT http://dkr.cdri.res.in:8080/xmlui/handle/123456789/1377 2013-01-01T00:00:00Z