http://dkr.cdri.res.in:8080/xmlui/handle/123456789/630 2026-04-19T13:41:21Z http://dkr.cdri.res.in:8080/xmlui/handle/123456789/637 Optimization of actinomycin V production by Streptomyces triostinicus using artificial neural network and genetic algorithm Singh, Vineeta; Khan, Mahvish; Khan, Saif; Tripathi, C K M Artificial neural network (ANN) and genetic algorithm (GA) were applied to optimize the medium components for the production of actinomycinV from a newly isolated strain of Streptomyces triostinicus which is not reported to produce this class of antibiotics. Experiments were conducted using the central composite design (CCD), and the data generated was used to build an artificial neural network model. The concentrations of five medium components (MgSO4, NaCl, glucose, soybean meal and CaCO3) served as inputs to the neural network model, and the antibiotic yield served as outputs of the model. Using the genetic algorithm, the input space of the neural network model was optimized to find out the optimum values for maximum antibiotic yield. Maximum antibiotic yield of 452.0 mg l−1 was obtained at the GA-optimized concentrations of medium components (MgSO4 3.657; NaCl 1.9012; glucose 8.836; soybean meal 20.1976 and CaCO3 13.0842 gl−1). The antibiotic yield obtained by the ANN/GA was 36.7% higher than the yield obtained with the response surface methodology (RSM). 2009-01-01T00:00:00Z http://dkr.cdri.res.in:8080/xmlui/handle/123456789/632 A monoclonal IgM directed against immunodominant catalase B of cell wall of Aspergillus fumigatus exerts anti-A. fumigatus activities Chaturvedi, A K; Kumar, Rohitashw; Kumar, Awanit; Shukla, P K Aspergillus fumigatus a ubiquitous fungus has been reported to cause human diseases like allergic pulmonary aspergillosis, aspergilloma, and invasive infection. Invasive aspergillosis is one of the most important causes of mortality in patients with hematological malignancies and in bone marrow transplant recipients. The currently available antifungal drugs mostly target the ergosterol synthesis. However, there are a few antifungals, such as echinocandin and glycolipid papulacandin, with which inhibition of cell wall glucans biosynthesis leads to cessation of growth and cell lysis. Limited spectrum and emergence of resistance has become a serious problem with available antifungals. Therefore, an alternative approach is required for successful treatment of mycoses. In the present study, immunogenic protein profile of A. fumigatus cell wall was generated using 2D-gel electrophoresis and three hybridomas producing monoclonal antibodies (IgM) were selected after fusion experiments. Of these three monoclonal antibodies, MAb-7 exhibited potent in vitro inhibitory activity, which was confirmed by MTT assay, FACS analysis, and immuno-fluorescence studies, and the protein was identified as catalase B using MALDI-TOF-MS. 2009-01-01T00:00:00Z http://dkr.cdri.res.in:8080/xmlui/handle/123456789/631 A monoclonal IgM directed against immunodominant catalase B of cell wall of Aspergillus fumigatus exerts anti-A. fumigatus activities Chaturvedi, A K; Kumar, Rohitashw; Kumar, Awanit; Shukla, P K Aspergillus fumigatus a ubiquitous fungus has been reported to cause human diseases like allergic pulmonary aspergillosis, aspergilloma, and invasive infection. Invasive aspergillosis is one of the most important causes of mortality in patients with hematological malignancies and in bone marrow transplant recipients. The currently available antifungal drugs mostly target the ergosterol synthesis. However, there are a few antifungals, such as echinocandin and glycolipid papulacandin, with which inhibition of cell wall glucans biosynthesis leads to cessation of growth and cell lysis. Limited spectrum and emergence of resistance has become a serious problem with available antifungals. Therefore, an alternative approach is required for successful treatment of mycoses. In the present study, immunogenic protein profile of A. fumigatus cell wall was generated using 2D-gel electrophoresis and three hybridomas producing monoclonal antibodies (IgM) were selected after fusion experiments. Of these three monoclonal antibodies, MAb-7 exhibited potent in vitro inhibitory activity, which was confirmed by MTT assay, FACS analysis, and immuno-fluorescence studies, and the protein was identified as catalase B using MALDI-TOF-MS. 2009-01-01T00:00:00Z