http://dkr.cdri.res.in:8080/xmlui/handle/123456789/58 2026-04-19T13:41:21Z 2026-04-19T13:41:21Z Dubey, C K Meena, A K Mishra, Vaibhav Singh, Poonam Bansode, F W Singh, R K http://dkr.cdri.res.in:8080/xmlui/handle/123456789/1676 2017-04-19T11:09:09Z 2015-01-01T00:00:00Z

Antioxidant And Haematoprotective Activity of The Saraca Indica Stem Bark Dubey, C K; Meena, A K; Mishra, Vaibhav; Singh, Poonam; Bansode, F W; Singh, R K Present study was designed to evaluate the antioxidant and hematoprotective activity of methanolic extract of saraca indica bark. Antioxidants and hematoprotective effect of saraca indica bark may be due to its polyphenolic compounds. Polyphenols act as reducing agents via scavenging of free radicals and chelation of transition metals. The hematoprotective activity of Saraca indica was studied in Phenylhydrazine induced anemic rats. Anemia was induced by an oral administration of Phenylhydrazine for a period of 7 days. Hgb concentration, RBC count, PCV, MCH, MCHC were analyzed as indices of anemia. Phenylhydrazine significantly decrease the Hgb, RBC and increased MCV and WBC. The Saraca indica was administered at the dose levels of 200mg/kg, 400mg/kg orally to the animals for 14 days. After 14 days of treatment with Saraca indica at the dose level of 400 mg/kg significantly reverse the above parameters and turn towards the normal value. These results support the traditional use of Saraca indica in the treatment of anemia.

2015-01-01T00:00:00Z Omer, Ankur Singh, Poonam Yadav, N K Singh, R K http://dkr.cdri.res.in:8080/xmlui/handle/123456789/1651 2016-06-24T11:38:15Z 2015-01-01T00:00:00Z

microRNAs: role in leukemia and their computational perspective Omer, Ankur; Singh, Poonam; Yadav, N K; Singh, R K MicroRNAs belong to the family of ncRNAs and had gained importance due to its role in complex biochemical pathways. Changes in the expression of protein coding genes are the major cause of leukemia. Role of miRNAs as tumor suppressors has provided a new insight in the field of leukemia research. Particularly, the miRNAs mediated gene regulation involves the modulation of multiple mRNAs and cooperative action of different miRNAs to regulate a particular gene expression. This highly complex array of regulatory pathway network indicates the great possibility in analysing and identifying novel findings. Owing to the conventional, slow experimental identification process of miRNAs and their targets, the last decade has witnessed the development of a large amount of computational approaches to deal with the complex interrelations present within biological systems. This article describes the various roles played by miRNAs in regulating leukemia and the role of computational approaches in exploring new possibilities.

2015-01-01T00:00:00Z Kumar, Akhilesh Chandasana, Hardik Bhatta, R S Sethi, Nikunj Yadav, Sudhaker Sinha, Neeraj http://dkr.cdri.res.in:8080/xmlui/handle/123456789/1614 2016-03-31T12:24:11Z 2015-01-01T00:00:00Z

Curcumin Affords Protection against Valproic Acid Induced Teratogenicity by Curtailing Oxidative Stress and Inhibiting CYP2C9 activity Kumar, Akhilesh; Chandasana, Hardik; Bhatta, R S; Sethi, Nikunj; Yadav, Sudhaker; Sinha, Neeraj Administration of drugs during pregnancy is always done with immense caution however, multiple neurological ailments including epilepsy and depression warrant medical treatments even during pregnancy. This exposes unborn fetus to killer teratogenic effects, thus warranting intense studies towards finding potential anti-teratogenic agents. We employed valproic acid (VPA) induced model of fetotoxicity and teratogenicity in rats towards assessing the antiteratogenic activity of curcumin, an antioxidant well known for attenuating oxidative stress by increasing the content of glutathione and reducing the level of lipid hydroperoxide. We studied the level of GSH, catalase, SOD, ROS, TBARS and activities of CYP2C9 and figured that VPA at the dose of 300 mg/kg body wt. significantly decreased the GSH, SOD, catalase and increased the levels of ROS, TBARS, mRNA expression and levels of CYP2C9 enzyme which is involved in the formation of toxic metabolite (E)-2,4-dieneVPA. Upon co-administration of curcumin (100,150 and 200 mg/kg body wt.) along with VPA the levels of GSH, SOD, catalase exhibited significant increase and ROS, TBARS, mRNA expression and level of CYP2C9 enzyme were found to be significantly decreased with respect to VPA. We conclude that the toxic metabolite (E)-2,4-dieneVPA is involved in generation of oxidative stress subsequently contributing in induction of malformations and anomalies and that curcumin affords dose dependent amelioration of the anomalies exerted by VPA. Our studies are suggestive of the fact that curcumin has antioxidant activity and can curtail the formation of toxic (E)-2, 4-dieneVPAby inhibiting CYP2C9 enzyme and finally protecting fetuses in dose dependent manner.

2015-01-01T00:00:00Z Fatima, Soobiya Haque, Rizwanul Jadiya, Pooja Shamsuzzama Kumar, Lalit Nazir, Aamir http://dkr.cdri.res.in:8080/xmlui/handle/123456789/1552 2015-06-05T06:11:52Z 2014-01-01T00:00:00Z

Ida-1, the Caenorhabditis elegans Orthologue of Mammalian Diabetes Autoantigen IA-2, Potentially Acts as a Common Modulator between Parkinson’s Disease and Diabetes: Role of Daf-2/Daf-16 Insulin Like Signalling Pathway Fatima, Soobiya; Haque, Rizwanul; Jadiya, Pooja; Shamsuzzama; Kumar, Lalit; Nazir, Aamir The lack of cure to age associated Parkinson’s disease (PD) has been challenging the efforts of researchers as well as health care providers. Recent evidences suggest that diabetic patients tend to show a higher future risk for PD advocating a strong correlation between PD and Diabetes, thus making it intriguing to decipher common genetic cues behind these ailments. We carried out studies on ida-1, the C. elegans orthologue of mammalian type-1 diabetes auto-antigen IA-2 towards achieving its functional workup vis-à-vis various associated endpoints of PD and Diabetes. Employing transgenic C. elegans strain expressing “human” alpha synuclein (NL5901) under normal and increased glucose concentrations, we studied aggregation of alpha synuclein, content of dopamine, expression of dopamine transporter, content of reactive oxygen species, locomotor activity, nuclear translocation of FOXO transcription factor Daf-16, and quantification of Daf2/Daf-16 mRNA. Our findings indicate that ida-1 affords protection in the studied disease conditions as absence of ida-1 resulted in higher alpha-synuclein aggregation under conditions that mimic the blood glucose levels of diabetic patients. We also observed reduced dopamine content, decreased motility, defective Daf-16 translocation and reduced expression of Daf-2 and Daf-16. Our studies establish important function of ida-1 as a modulator in Daf-2/Daf-16 insulin like signalling pathway thus possibly being a common link between PD and Diabetes.

2014-01-01T00:00:00Z