http://dkr.cdri.res.in:8080/xmlui/handle/123456789/1372 2026-04-19T13:48:36Z 2026-04-19T13:48:36Z Singh, Awantika Bajpai, Vikas Srivastava, Mukesh Arya, K R Kumar, Brijesh http://dkr.cdri.res.in:8080/xmlui/handle/123456789/1681 2017-04-28T10:18:14Z 2015-01-01T00:00:00Z

Rapid screening and distribution of bioactive compounds in different parts of Berberis petiolaris using direct analysis real time mass spectrometry Singh, Awantika; Bajpai, Vikas; Srivastava, Mukesh; Arya, K R; Kumar, Brijesh Berberis petiolaris Wall. ex G. Don, a less known medicinal plant belonging to family Berberidaceae locally known as Kingora or Kilmora is a large deciduous shrub found in Western Himalaya between 1800–3000 m. Decoction of stem and root is used for treatment of malarial fever, diahhorea, conjunctivitis and also jaundice. Chemical profiling of fruit, leaf, root and stem were done using DART TOF MS. The bioactive compounds magnoflorine, berberine, jatrorrhizine, thalifendine/berberrubine, demethyleneberberine, reticuline, 8-oxoberberine, N-methyltetrahydroberberine, tetrahydropalmatine, tetrahydroberberine and palmatine were identified by their exact mass measurement and the corresponding molecular formula of each compound. A comparative study of distribution pattern for all these bioactive alkaloids showed qualitative and quantitative variation in different parts of B. petiolaris. PCA with multivariate analysis clearly discriminated each part of B. petiolaris plant.

2015-01-01T00:00:00Z Singh, Awantika Bajpai, Vikas Srivastava, Mukesh Arya, KR Kumar, Brijesh http://dkr.cdri.res.in:8080/xmlui/handle/123456789/1659 2017-03-17T06:00:49Z 2014-01-01T00:00:00Z

Rapid profiling and structural characterization of bioactive compounds and their distribution in different parts of Berberis petiolaris Wall. ex G. Don applying hyphenated mass spectrometric techniques Singh, Awantika; Bajpai, Vikas; Srivastava, Mukesh; Arya, KR; Kumar, Brijesh RATIONALE: Berberis petiolaris Wall., is a lesser known medicinal plant, belonging to family Berberidaceae. Genus berberis is known for many biological activities such as anti-microbial, anti-inflammatory and anti-diarrheal etc. There are not many reports of isolation of components from Berberis petiolaris. This study aims to seek identification, characterization and quantification of components. METHODS: A method was developed for rapid screening of phytochemicals using high-pressure liquid chromatography hyphenated with quadrupole time-of-flight mass spectrometer (HPLC-ESI-QTOF-MS/MS). Suitable collision-induced dissociation mass spectrometry (CID-MS/MS) method was developed for structural investigation of different class of alkaloids, flavanoids and other class of compounds in which nine standard compounds were used for authentication. Multiple reaction monitoring (MRM) method was developed for quantitative study of five constituents using triple quadrupole-linear ion trap mass spectrometer (UPLC-QTRAP-MS). RESULTS: On the basis of HPLC retention behaviour and fragmentation pathways obtained by high-resolution MS and MS/MS spectra, 32 compounds were identified and characterized in different parts of Berberis petiolaris. Quantitative study of chlorogenic acid, magnoflorine, jatrorrhizine, palmatine and berberine were also completed successfully. CONCLUSIONS: Rapid and accurate HPLC-DAD/ESI-QTOF-MS/MS and UPLC-QTRAP-MS methods were established for identification, characterization and quantification of phytochemicals in the ethanolic extract of Berberis petiolaris. These methods therefore can be used for studies on phytochemical variation in different parts of the plant. PCA analysis may be used for plant part discrimination.

2014-01-01T00:00:00Z Kathuria, Manoj Bhattacharjee, Arindam Sashidhara, K V Singh, S P Mitra, Kalyan http://dkr.cdri.res.in:8080/xmlui/handle/123456789/1650 2016-06-24T10:39:45Z 2014-01-01T00:00:00Z

Induction of mitochondrial dysfunction and oxidative stress in Leishmania donovani by orally active clerodane diterpene Kathuria, Manoj; Bhattacharjee, Arindam; Sashidhara, K V; Singh, S P; Mitra, Kalyan This study was performed to investigate the mechanistic aspects of cell death induced by a clerodane diterpene (K-09) in Leishmania donovani promastigotes previously demonstrated to be safe and orally active against Visceral Leishmaniasis (VL). K-09 caused depolarization of the mitochondrion and generation of reactive oxygen species triggering an apoptotic response in L. donovani promastigotes. Mitochondrial dysfunction subsequently resulted in release of cytochrome c into the cytosol impairing ATP production. Oxidative stress caused depletion of reduced glutathione while pre-treatment with anti-oxidant N-acetyl-cysteine (NAC) was able to abrogate oxidative stress. However, NAC failed to restore mitochondrial membrane potential or intracellular calcium homeostasis after K-09 treatment suggesting that generation of oxidative stress is a downstream event relative to the other events. Caspase-3/7-like protease activity and genomic DNA fragmentation were observed. Electron microscopic studies revealed gross morphological alterations typical of apoptosis, including severe mitochondrial damage, pyknosis of nucleus, structural disruption of the mitochondrion-kinetoplast complex, flagellar pocket alterations and displacement of organelles. Moreover, increased number of lipid droplets was detected after K-09 treatment which is suggestive of altered lipid metabolism. Our results indicate that K-09 induces mitochondrial dysfunction and oxidative stress mediated apoptotic cell death in L. donovani promastigotes sharing many features with metazoan apoptosis. These mechanistic insights provide a basis for further investigation towards development of K-09 as a potential drug candidate for VL.

2014-01-01T00:00:00Z Pandey, Renu Chandra, Preeti Srivastva, Mukesh Arya, K R Shukla, P K Kumar, Brijesh http://dkr.cdri.res.in:8080/xmlui/handle/123456789/1616 2016-04-11T11:55:43Z 2014-01-01T00:00:00Z

A Rapid Analytical Method for Characterization and Simultaneous Quantitative Determination of Phytoconstituents in Piper betle landraces Using UPLC-ESI-MS/MS Pandey, Renu; Chandra, Preeti; Srivastva, Mukesh; Arya, K R; Shukla, P K; Kumar, Brijesh A rapid ultra performance liquid chromatography electrospray ionisation tandem mass spectrometry (UPLC-ESI-MS/MS) method was developed and validated for identification and characterization of phytoconstituents with simultaneous quantitative determination of three major bioactive phenolics namely allylpyrocatechol-3, 4-diacetate, eugenyl acetate and eugenol from thirteen landraces of Piper betle leaf extracts. Among the 29 phytoconstituents detected, 19 were identified and characterized based on their fragmentation pattern obtained via MS/MS by means of collision-induced-dissociation (CID) and by comparison of their retention time with authentic standards or by previously reported literature. The proposed method was fully validated in terms of linearity, LOD, LOQ, precision, stability and recovery. All calibration curves showed a good linear relationship (r2 ≥ 0.9981). The precision evaluated by an intra- and inter-day study showed RSDs ≤ 1.0% and good accuracy with overall recovery in the range from 96.14 – 98.46% (RSD ≤ 1.6%) for all analytes. The quantitative results showed that there were remarkable differences in the contents of the major bioactive phenolics in all the thirteen landraces of P. betle. The developed UPLC-ESI-MS/MS method was found to be simple, precise, sensitive and accurate. Hence, it can be reliably utilized as a quality control method for P. betle or derived herbal formulations. In vitro antimicrobial activity of crude extract of P. betle landraces were also evaluated, all extracts tested exhibited antifungal activity but none of the extracts were found to be active against bacteria even at 500 µg mL1 concentrations.

2014-01-01T00:00:00Z